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Core Analytical Laboratory

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University at Buffalo, the State University of New York School of Pharmacy and Pharmaceutical Sciences

HPLC

Tipranavir

Antiretrovirals

Nevirapine and Abacavir

Sumitriptan

Delavirdine and its Metabolite

Efavirenz

Free Phenytoin

New assays in development:

darunavir assay, which will include atazanavir, efavirenz, lopinavir, saquinavir, and ritonavir
nevirapine, lamivudine and stavudine

Antiretrovirals

Simultaneous HPLC Method for Protease Inhibitors Plus Efavirenz
	Analyte	Lower Limit of Quantitation(μg/L)	Acceptable Anticoagulants	Required Plasma Volume	nM
Assay 1	Amprenavir	100	K3-EDTA or Sodium Heparin	Minimal 0.6 mL	265
	Atazanavir	50			248
	Efavirenz	100			248
	Lopinavir	200			215
	Ritonavir	200			215
	Saquinavir	100			235
Assay 2	Indinavir	100	K3-EDTA or Sodium Heparin	Minimal 0.6 mL	215
	Nelfinavir	100			215
	Nelfinavir's Active Metabolite	100			215

An isocratic reverse phase high performance liquid chromatography method measuring efavirenz and protease inhibitor compounds (amprenavir (APV), atazanavir (ATV), nelfinavir (NFV), indinavir (IDV), saquinavir (SQV), ritonavir (RTV), nelfinavir's metabolite M8, and lopinavir (LPV)) in heparinized human plasma has been validated at The Laboratory for Antiviral Research at The State University of New York at Buffalo. After the addition of internal standard, plasma specimens are prepared for chromatographic analysis via liquid-liquid extraction. The organic phase from the sample preparation is evaporated to dryness, reconstituted in mobile phase and injected into the reverse phase chromatographic system. Compounds are detected at various UV wavelengths using a photodiode array detector. The lower limit of quantitation ranges from 50 to 200 ng/mL.