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Core Analytical Laboratory

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University at Buffalo, the State University of New York School of Pharmacy and Pharmaceutical Sciences

HPLC

Tipranavir

Antiretrovirals

Nevirapine and Abacavir

Sumitriptan

Delavirdine and its Metabolite

Efavirenz

Free Phenytoin

New assays in development:

darunavir assay, which will include atazanavir, efavirenz, lopinavir, saquinavir, and ritonavir
nevirapine, lamivudine and stavudine

Free Phenytoin

Analyte

Lower Limit of Quantitation

(ng/mL)

Required Sample Volume

Wavelength

Phenytoin

100

Minimal 50µL

215 nm

An isocratic reverse phase high performance liquid chromatography method measuring free phenytoin in ultra filtrate has been developed and validated at Pharmacotherapy Research Center's Core Analytical Laboratory. After the addition of internal standard, 6, 7-dimethyl-2, 3-di (2-pydridyl) quinoxaline, ultra filtrate specimens are prepared for chromatographic analysis via evaporation. Samples are reconstitution with mobile phase and injected into the chromatographic system. Phenytoin and the internal standard are detected by UV detection at a wavelength of 215 nm. The lower limit of quantitation is 100 ng/mL.