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New assays in development:

• darunavir assay, which will include atazanavir, efavirenz, lopinavir, saquinavir, and ritonavir
• nevirapine, lamivudine and stavudine

Nevirapine and Abacavir

An isocratic reverse phase high performance liquid chromatography method measuring nevirapine and abacavir heparinized human plasma has been adopted and validated at The Laboratory for Antiviral Research at The State University of New York at Buffalo. This method was first developed and validated by the University at Alabama; only the internal standard was changed when the method was validated at Buffalo. After the addition of internal standard, chloramphenicol, plasma specimens are prepared for chromatographic analysis via liquid-liquid extraction. The organic phase from the sample preparation is evaporated to dryness, reconstituted in mobile phase and injected into the reverse phase chromatographic system. Compounds are detected at a wavelength setting of 284 using a UV detector. The lower limit of quantitation is 250 ng/mL.